Approaches to promoting bone marrow mesenchymal stem cell osteogenesis on orthopedic implant surface
نویسندگان
چکیده
منابع مشابه
INSTANT STEM CELL THERAPY FOR ORTHOPEDIC APPLICATIONS: Automated separation of mesenchymal cells from bone marrow aspirates
INTRODUCTION: Mesenchymal stem cells (MSC) constitute an essential element in many applications in regenerative medicine. In orthopedics, they are used as osteoinductive component for bone regeneration. One source for MSC is bone marrow (BM) aspirate which contains concentrations of 10-100 MSC per Mio BM cells. This low number requires an efficient isolation procedure and usually an in-vitro ex...
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BACKGROUND/AIMS We investigated the role of dimethyloxalylglycine (DMOG) in bone marrow mesenchymal stem cell (BMSC) osteogenesis mediated by RhoA/ROCK. METHODS BMSCs were cultured with and without DMOG and/or Y-27632 (ROCK1 inhibitor). Cell proliferation, alkaline phosphatase (ALP) levels, and calcium deposits were determined. The expression of Runx2, OSX, p-cofilin, RhoA, and GTP-bound RhoA...
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ژورنال
عنوان ژورنال: World Journal of Stem Cells
سال: 2020
ISSN: 1948-0210
DOI: 10.4252/wjsc.v12.i7.545